Metallomics, Lipidomics, Assessment of drug quality and safety, Study on the material basis and mechanisms of TCMs

1. Research Projects

(1)Study of the mechanisms of cinnabar-induced central neurotoxicity based on the Metallomics of mercury (Grant Number: 81903911), supported by National Natural Science Foundation of China, 2020-2022.

(2)Study of the central neurotoxicity of Cinnabaris based on the Metallomics (Grant Number: BK20190556), supported by Natural Science Foundation of Jiangsu Province, 2019- .

(3)Study on the toxicity and safety of Cinnabaris-containing TCM Baizi Yangxin Pill based on the mercury speciation analysis (Grant Number: 2017M621885), supported by China Postdoctoral Science Foundation, 2017-2020.

2. Representative Research Achievements

(1)Impurity analysis is one of the key and difficult points in drug quality control. Based on the modern analytical techniques including HRMS, NMR, a drug impurity research strategy has been established. It has helped a series of manufacturers to optimize the production process and improve the quality of API and pharmaceutics such as flumazenil and zopiclone.

(2)Cinnabar (HgS) is a mercury-containing traditional Chinese material medica with potential toxicity. Our group studied on the mechanisms of the cinnabar-induced central neurotoxicity through the Metallomics and Lipidomics methods. Combined with the results of LA-ICP-MS in situ imaging of mercury in rat’s brain, HPLC-CV-AFS mercuric species, LC-HRMS lipid analysis, the material basis and the mechanisms of cinnabar-induced central neurotoxicity were revealed, which can provide scientific references for the safety evaluation and rational use of cinnabar.

1. Wu W.; Cheng R.; Boucetta H.; Xu L.; Pan J.R.; Song M.; Lu, Y.T.*; Hang, T.J.*. Differences in Multicomponent Pharmacokinetics, Tissue Distribution, and Excretion of Tripterygium Glycosides Tablets in Normal and Adriamycin-Induced Nephrotic Syndrome Rat Models and Correlations with Efficacy and Hepatotoxicity. Front. Pharmacol. 2022, 13, 910923. (IF: 5.988)

2. Jiang, J.; Hu, Z.L.; Boucetta, H.; Liu, J.M.; Song, M.; Hang, T.J.*; Lu, Y.T.* Identification of degradation products in flumazenil using LC-Q-TOF/MS and NMR: Degradation pathway elucidation.  J. Pharm. Biomed. Anal. 2022, 12;215:114764. (IF: 3.571)

3. Xu, L.; Wang, Y.; Wu, D.F.; Sun, F.M.; Di, B.; Xu, H.; Song, M.; Lu, Y.T.*; Hang, T.J.* Identification and monitoring of fentanyls-related substances in east China sewage water samples by LC-MS for drug enforcement. Sci Total Environ. 2021, 797:149109. (IF:7.963)

4. Han, X.; Cheng, F.J.; Di, B.; Xu, H.; Song, M.; Hang, T.J.*; Lu, Y.T.* Identification and characterization of new impurities in zopiclone tablets by LC-QTOF-MS. J. Pharm. Biomed. Anal. 2021, 199:114056. (IF: 3.935)

5. Lu, Y.T.; Hu, Z.L.; Shao, F.X.; Song, M. Hang, T.J.* Simultaneous determination of tazarotene, clindamycin phosphate and their active metabolites in Bama mini-pig skin by LC-MS/MS: Application to the development of a tazarotene/clindamycin phosphate cream techniques. J. Chromatogr. B 2021, 1162:122455. (IF: 3.205)

6. Lu, Y.T.; Guan, R.; Zheng, J.R.; Hang, T.J.*; Song, M.* Determination of clobetasol propionate, tazarotene and tazarotenic acid in Bama mini-pig skin by UPLC-MS/MS: Application to pharmacokinetic and drug-drug interaction studies. J. Pharm. Biomed. Anal. 2020, 186, 113322. (IF: 3.209)

7. Lu, Y.T.; Qi, W.Z.; Wang, S.; Song, X.N.; Yang, D.Y.; Song, M.; Hang, T.J.* Toxicity and risk assessment of mercury exposures from cinnabar and Baizi Yangxin Pills based on pharmacokinetic and tissue distribution studies. J Ethnopharmacol. 2020, 250, 112489. (IF: 4.360)

8. Lu, Y.T.; Chen, Y.F.; Qi, W.Z.; Song, M.*; Hang, T.J.* Separation and identification of process-related substances and degradation products in mycophenolate mofetil by LC-QTOF mass spectrometric method. J. Sep. Sci. 2019, 42(16):2650-2659. (IF: 2.878)

9. Lu, Y.T.; Sun, X.Y.; Song, F.; Wang, L.; Song, M.; Hang, T.J.* Impurity profiling of varenicline tartrate by LC-QTOF mass spectrometric techniques during drug development. J. Pharm. Biomed. Anal. 2018, 155: 306-313. (IF: 2.983)

10. Lu, Y.T.; Yang, D.Y.; Song, X.N.; Wang, S.; Song, M.; Hang, T.J.* Bioaccessibility and health risk assessment of mercury in cinnabar containing Traditional Chinese Medicines. J. Trace Elem. Med. Bio. 2017, 44: 17-25. (IF: 3.755)